Thermo Fisher Scientific PHF7 Monoclonal Antibody (OTI1A11), TrueMAB
PHF7 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI1A11). Western blot, IHC, ICC, Flow cytometry에 사용 가능. Human 시료 반응성. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1A11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 155–381 of human PHF7 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Spermatogenesis is regulated by extracellular and intracellular factors and by cellular interactions among interstitial cells, Sertoli cells, and germ cells.
This gene is expressed in Sertoli cells but not in germ cells. Lack of expression has been observed in patients exhibiting spermatogenic arrest at the spermatocyte stage, which may lead to oligospermia or azoospermia.
The encoded proteins contain plant homeodomain (PHD) finger domains (also known as leukemia associated protein, LAP domains) involved in transcriptional regulation.
This protein localizes to the nucleus of transfected cells and is implicated in transcriptional regulation of spermatogenesis. Two isoforms are encoded by transcript variants of this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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