Thermo Fisher Scientific MERTK Monoclonal Antibody (DS5MMER), Functional Grade, eBioscience
Mouse MerTK 인식 단클론 항체로, 기능적 등급의 eBioscience 제품입니다. Flow cytometry, WB, IHC 등 다양한 응용에 사용 가능하며 Gas6 유도 MerTK 인산화를 억제합니다. 1 mg/mL 농도의 액상 형태로 4°C에서 보관합니다.
- 카탈로그번호
- 16-5751-xxx (4개 옵션)
- 판매단위
- pk
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4개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific MERTK Monoclonal Antibody (DS5MMER), Functional Grade, eBioscience
Thermo Fisher Scientific MERTK Monoclonal Antibody (DS5MMER), Functional Grade, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | 5 µg/mL | - |
| Flow Cytometry (Flow) | Assay-Dependent | View 6 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Functional Assay (Functional) | Assay-Dependent | - |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DS5MMER |
| Immunogen | FC-tagged full protein expressed in insect cells |
| Conjugate | Functional Grade |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2866034 |
Available Conjugated Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant UV 395
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 480
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
This DS5MMER monoclonal antibody recognizes mouse MerTK, a 170–210 kDa member of the TAM family of tyrosine kinase receptors that includes Axl and Tyro3. MerTK is expressed on tissue macrophages and mediates apoptotic cell clearance via Protein S and Gas6 ligands. Ligand binding induces MerTK autophosphorylation, activating PI3K and Akt pathways, leading to phagocytosis and inhibition of TLR-induced cytokine production. MerTK may also serve as an entry receptor for filoviruses, and its deficiency leads to autoimmunity and inflammation. It is constitutively shed from the cell surface by metalloproteinases.
Applications Reported: Flow cytometry, IHC (frozen), WB, IP, and functional assays.
Applications Tested: Inhibits Gas6-induced MerTK phosphorylation (IC₅₀ < 5 nM).
Recommended Concentration: ≤5 µg/mL for IHC (frozen).
Storage and Handling: Use in sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90% (SDS-PAGE).
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10% (HPLC).
Target Information
MERTK (cMER) is a tyrosine kinase proto-oncogene involved in retinal pigment epithelium (RPE) phagocytosis and various physiological processes such as cell survival, migration, differentiation, and efferocytosis. Ligand-induced autophosphorylation recruits signaling molecules like GRB2 and PLCG2, activating MAPK1/2, FAK/PTK2, and RAC1. MERTK signaling contributes to macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization, and inhibition of TLR-mediated immune responses via STAT1 activation and SOCS1/3 induction.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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