Thermo Fisher Scientific CD244 Monoclonal Antibody (eBioC1.7 (C1.7)), Functional Grade, eBioscience

Thermo Fisher Scientific CD244 Monoclonal Antibody (eBioC1.7 (C1.7)), Functional Grade

Applications and Tested Dilutions

Product Specifications

Available Conjugate Formats

• Biotin
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• PE
• PE-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The eBioC1.7 monoclonal antibody reacts with human CD244 (2B4, p38). CD244 is a 38 kDa protein expressed on NK cells, a subset of CD8+ T cells, γδ T cells, monocytes, basophils, and eosinophils. Binding of CD244 to its ligand CD48 results in NK cell activation and increased cytotoxicity and IFN-γ secretion.

Binding of the C1.7 monoclonal antibody and CD48 involves the same residue in the V domain of human CD244, explaining the activation of NK cells upon C1.7 binding. This interaction leads to tyrosine phosphorylation and recruitment of the adaptor molecule SAP (SLAM-associated protein). Patients with X-linked lymphoproliferative disorder (XLPD) have a mutation in SAP that prevents its binding to phosphorylated CD244.

Applications Reported: Flow cytometric analysis and Western blot (WB).
Applications Tested: Flow cytometric analysis of normal human peripheral blood. Recommended ≤0.5 µg/test (100 µL final volume, 10^5–10^8 cells/test). Titrate for optimal performance.

Storage and Handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: >90%, determined by SDS-PAGE.
Endotoxin Level: <0.001 ng/µg antibody (LAL assay).
Aggregation: <10%, determined by HPLC.

Target Information

CD244 (2B4, SLAMF4) is a cell surface receptor on NK cells and some T cells that mediates non-MHC restricted killing. Interaction with SLAMF2 modulates NK-cell activity. CD244 dysfunction is associated with autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis, and lymphoproliferative syndrome. Alternative splicing produces multiple isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.