Thermo Fisher Scientific CD47 Recombinant Rabbit Monoclonal Antibody (BLR131H)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Gel Shift (GS)

1:10-1:250

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293F

Class

Recombinant Monoclonal

Type

Antibody

Clone

BLR131H

Immunogen

Recombinant protein representing the extracellular domain of Human leukocyte surface antigen CD47 (residues 19-142). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD47,uniProtId:Q08722-1,ncbiNodeId:9606,antigenRange:19-142,antigenLength:323,antigenImageFileName:A700-131_CD47_Q08722-1_Rabbit.svg,antigenImageFileNamePDP:A700-131_CD47_Q08722-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

50 µg/mL

Purification

purified

Storage buffer

BBS, pH 8.2, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Product is tested in SW-Size.

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.