Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) ReadyProbes Secondary Antibody, Alexa Fluor 594
Alexa Fluor 594 형광 표지를 가진 Donkey anti-Mouse IgG (H+L) ReadyProbes 2차 항체로, 희석 없이 즉시 사용 가능. 세포 염색 및 형광 이미징에 적합하며, 비특이적 반응 최소화. 590/618 nm 파장에서 최적의 형광 반응 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 drops per mL |
| Immunocytochemistry (ICC/IF) | 2 drops per mL |
| Flow Cytometry (Flow) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobin |
| Conjugate | Alexa Fluor™ 594 |
| Excitation / Emission Max | 590 / 618 nm |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 0.1% BSA |
| Contains | <0.1% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556543 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 488
- AP
- Biotin
- FITC
- HRP
- Qdot 525 / 605 / 625
- Texas Red
- TRITC
- Custom conjugation available upon request
Product Specific Information
- Ready-to-use format: no pipetting or dilution required.
- Use two drops per mL of buffer for staining.
- Shows minimal cross-reactivity with bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins.
- Supplied in a convenient dropper bottle for easy handling.
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of mouse antibodies and compatible with various detection systems (HRP, AP, fluorescence).
Secondary antibodies enhance sensitivity via signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
They are typically generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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