Thermo Fisher Scientific Donkey anti-Mouse IgG (H+L) ReadyProbes Secondary Antibody, Alexa Fluor 488

Applications

Tested Dilution

Western Blot (WB)

2 drops per mL

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunocytochemistry (ICC/IF)

2 drops per mL

Flow Cytometry (Flow)

Assay-Dependent

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Donkey / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobin

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• [Alexa Fluor 594 ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Alexa Fluor 594/host/Donkey/)
• [AP](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/AP/host/Donkey/)
• [Biotin ](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Biotin/host/Donkey/)
• [FITC](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/FITC/host/Donkey/)
• [HRP](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/HRP/host/Donkey/)
• [Qdot 525](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 525/host/Donkey/)
• [Qdot 605](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 605/host/Donkey/)
• [Qdot 625](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Qdot 625/host/Donkey/)
• [Texas Red](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/Texas Red/host/Donkey/)
• [TRITC](/antibody/secondary/product-name/Mouse IgG (H--L)/conjugates/TRITC/host/Donkey/)
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS with 0.1% BSA

Contains

<0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2556542

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

No pipetting or dilutions required. Just tip and drip two drops per mL buffer to stain your cells.

This antibody shows minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins. Like other ReadyProbes reagents, Alexa Fluor 488 Donkey anti-Mouse IgG Antibody comes in a convenient dropper bottle.

Target Information

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.