Himedia Nutrient Mixture F-12 Ham, Kaighn`s Modification w/o L-Glutamine and Sodium bicarbonate

Himedia Nutrient Mixture F-12 Ham, Kaighn`s Modification w/o L-Glutamine and Sodium bicarbonate

Ham’s Nutrient Mixture F-12 was originally designed for serial propagation and cloning of two CHO cell lines, CHD-3 and CHL-1, and mouse L cells.
It is the medium of choice for the growth of cells of rodent origin and for cloning of myeloma and hybridoma cells.
This medium is also used for clonal toxicity assays employing CHO cells.

Ham’s modification by Kaighn’s and Konisberg, incorporating increased amounts of amino acids, pyruvate, and salts, favors the growth and differentiation of rat and chicken cells as well as primary human liver cells.

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