Thermo Fisher Scientific EGF Monoclonal Antibody (3A8)

Applications and Tested Dilution

Product Specifications

Additional Formats

• Biotin
• Request custom conjugation

Product Specific Information

The M806 anti-EGF antibody (Clone 3A8) has successfully been paired as the detection antibody in a sandwich ELISA with coating antibody M805 (Clone 1H11).
Typical dilutions for sandwich ELISA include 1–3 µg/mL for coating and 0.125–0.5 µg/mL for detection.

Target Information

Epidermal Growth Factor (EGF) binds to the EGF receptor (EGFR), a 170 kDa protein kinase. Activation of EGFR triggers diverse cellular pathways in response to environmental stimuli. Upon EGF binding, EGFR forms homo- or heterodimers with other family members, initiating distinct signaling cascades via recruitment of SH2-containing effector proteins.
EGF is widely expressed in kidney, cerebrum, prostate, and salivary glands, acting as a potent mitogenic factor. The phosphorylated receptor recruits adapter proteins such as GRB2, activating downstream signaling pathways including RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT.
Research indicates EGF may also activate NF-κB signaling. Defects in the EGF gene are associated with hypomagnesemia type 4, and dysregulation has been linked to certain cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.