Thermo Fisher Scientific Smoothelin Polyclonal Antibody
Smoothelin 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P)에서 검증됨. 완전 분화된 평활근세포(SMC) 표지에 유용하며, 항원 친화 크로마토그래피로 정제. PBS/glycerol buffer에 보관되며 연구용으로만 사용 가능.
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- PA563590
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- pk
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human Smoothelin. Recombinant protein control fragment (Product # RP-103336) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2647657 |
Product Specific Information
Immunogen sequence:
PVARSEEPGA PLPVAVGTAE PGGSMKTTFT IEIKDGRGQA STGRVLLPTG NQRAELTLGL RAPPTLLSTS SGGKSTITRV NSPGT
Highest antigen sequence identity to the following orthologs:
- Mouse: 86%
- Rat: 86%
Target Information
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are a useful tool to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein.
Cells with SMC-like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin and vimentin. Smoothelin colocalizes with actin stress fibers.
Two tissue-specific isoforms have been identified:
- 59 kDa isoform specific for visceral SMC
- 100 kDa isoform specific for vascular SMC
Human smoothelin is encoded by a single copy gene located on chromosome 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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