Thermo Fisher Scientific Smoothelin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human Smoothelin. Recombinant protein control fragment (Product #RP-103336). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Smoothelin,uniProtId:P53814-1,ncbiNodeId:9606,antigenRange:433-517,antigenLength:917,antigenImageFileName:PA5-63590_Smoothelin_P53814-1_Rabbit.svg,antigenImageFileNamePDP:PA5-63590_Smoothelin_P53814-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2647657

Product Specific Information

Immunogen sequence: PVARSEEPGA PLPVAVGTAE PGGSMKTTFT IEIKDGRGQA STGRVLLPTG NQRAELTLGL RAPPTLLSTS SGGKSTITRV NSPGT

Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 86%.

Target Information

Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are a useful tool to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin and vimentin. Smoothelin colocalizes with actin stress fibers. Two tissue specific isoforms have been identified: a 59 kDa isoform specific for visceral SMC; and a 100 kDa specific for vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.