Thermo Fisher Scientific SAP (SLAM-Associated Protein) Monoclonal Antibody (10C4.2), eBioscience

Thermo Fisher Scientific SAP (SLAM-Associated Protein) Monoclonal Antibody (10C4.2), eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

This 10C4.2 monoclonal antibody reacts with human SLAM-associated protein (SAP). Upon SLAM receptor ligation, this 16-kDa SH2 domain-containing adaptor protein recruits Fyn tyrosine kinase, which phosphorylates the receptor to activate downstream signaling events.
SAP is expressed in T, NK, and NKT cells, as well as eosinophils and platelets. It has also been detected in B cells and tumor cell lines such as Jurkat and Raji.
Studies demonstrate that SAP expression in T follicular helper cells is required for germinal center formation. Moreover, SAP is involved in NKT cell development, Th1/Th2 differentiation, and controlling NK cytotoxicity.
Deletion of the gene encoding SAP, SH2D1A, results in X-linked lymphoproliferative disease in humans.

Applications Reported: Immunoblotting and Immunocytochemistry (ICC)
Applications Tested: Immunoblotting of reduced Jurkat cell lysates, ≤5 µg/mL recommended.
Careful titration is advised for optimal assay performance.

Target Information

This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells.
It contains an SH2 domain and a short tail, and associates with the signaling lymphocyte-activation molecule (SLAM), acting as an inhibitor by blocking recruitment of SHP-2 to its docking site.
This protein also binds to other surface molecules on activated T, B, and NK cells, modifying signal transduction pathways.
Mutations in this gene cause X-linked lymphoproliferative syndrome type 1 (Duncan disease), a rare immunodeficiency characterized by susceptibility to Epstein-Barr virus infection, severe mononucleosis, and malignant lymphoma.
Multiple transcript variants encoding different isoforms have been identified.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.