Thermo Fisher Scientific Lipoamide Dehydrogenase Polyclonal Antibody
Thermo Fisher의 Lipoamide Dehydrogenase Polyclonal Antibody는 돼지 심장 유래 항원을 사용한 토끼 IgG 항체로, WB, ELISA, IP에 적합합니다. 고순도 이온 교환 크로마토그래피로 정제되었으며, 연구용으로 안정적이고 다양한 조직 반응성을 제공합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Lipoamide Dehydrogenase (Porcine Heart) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Lipoamide Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by delipidation, salt fractionation, and ion-exchange chromatography, followed by extensive dialysis.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase (Porcine Heart). Cross-reactivity against Lipoamide Dehydrogenase from other tissues and species may occur but has not been specifically determined.
Target Information
This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also known as dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex.
Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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