Thermo Fisher Scientific Phospho-TRKB (Tyr516) Polyclonal Antibody
Phospho-TRKB (Tyr516) 폴리클로날 항체로, 인간·마우스·랫트 시료에서 인산화된 TrkB를 특이적으로 검출. WB, IHC(P), ICC/IF에 적합하며, 1mg/mL 농도의 액상 제품. 신경계 발달 및 시냅스 기능 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NTRK2 (Accession Q16620), corresponding to amino acid residues around phosphorylated Tyr516. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816486 |
Product Specific Information
This antibody detects endogenous levels of TrkB only when phosphorylated at Tyrosine 516.
Target Information
NTRK2 (TRKB) is a member of the neurotrophic factor receptor family, crucial for the development and maintenance of the mammalian nervous system. It serves as the receptor for brain-derived neurotrophic factor (BDNF). Together, NTRK2 and BDNF regulate both short-term synaptic functions and long-term potentiation of brain synapses.
TrkB is a membrane-bound receptor that, upon neurotrophin binding, undergoes autophosphorylation and activates the MAPK signaling pathway, leading to cell differentiation. Mutations in the TrkB gene have been linked to obesity and mood disorders. Alternative splice variants encoding different isoforms are known.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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