Thermo Fisher Scientific MED30 Monoclonal Antibody (OTI3B3), TrueMAB
Human MED30 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot에 적합합니다. 무보존제, 동결건조 형태이며 재구성 후 1 mg/mL 농도로 사용 가능합니다. Pol II 매개 복합체 연구용으로 RNA 전사 조절 기전 분석에 활용됩니다.
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Applications
- Western Blot (WB)
Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B3 |
| Immunogen | Full length human recombinant protein of human MED30 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform desalting using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
Med30 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex.
This complex acts as a coactivator in regulated transcription of Pol II-dependent genes, bridging gene-specific regulatory proteins and the basal Pol II transcription machinery.
It is recruited to promoter regions through direct interaction with regulatory proteins and serves as a scaffold for assembly of a functional pre-initiation complex with Pol II and other transcription factors.
Med30 is localized in the nucleus and expressed in brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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