Thermo Fisher Scientific F(ab`)2-Goat anti-Rat IgG (H+L) Secondary Antibody, FITC, eBioscience

Applications

Tested Dilution

Flow Cytometry (Flow)

0.25 µg/test

Product Specifications

Species Reactivity

Rat

Host/Isotype

Goat / Ig

Class

Polyclonal

Type

Secondary Antibody

Conjugate

FITC FITC FITC

View additional formats

• [AP](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/Biotin/host/Goat/)
• [eFluor 660](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/eFluor 660/host/Goat/)
• [HRP](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/HRP/host/Goat/)
• [NovaFluor Blue 510](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 510/host/Goat/)
• [NovaFluor Blue 555](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 555/host/Goat/)
• [NovaFluor Blue 585](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 585/host/Goat/)
• [NovaFluor Blue 610-70S](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 610-70S/host/Goat/)
• [NovaFluor Blue 660-120S](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Blue 660-120S/host/Goat/)
• [NovaFluor Yellow 590](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Yellow 590/host/Goat/)
• [NovaFluor Yellow 610](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Yellow 610/host/Goat/)
• [NovaFluor Yellow 660](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/NovaFluor Yellow 660/host/Goat/)
• [PE ](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/PE/host/Goat/)
• [PE-Cyanine5](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/PE-Cyanine5/host/Goat/)
• [Qdot 605](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/Qdot 605/host/Goat/)
• [Qdot 655](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/Qdot 655/host/Goat/)
• [Rhodamine](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/Rhodamine/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/TRITC/host/Goat/)
• [WesternDot 625](/antibody/secondary/product-name/Rat IgG (H--L)/conjugates/WesternDot 625/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572491

Target

IgG

Antibody Form

F(ab`)2 Fragment

Product Specific Information

Description: The goat anti-rat IgG polyclonal antibody reacts with the heavy and light chains of rat IgG, with minimal cross reactivity to human, mouse, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to all IgG subclasses: IgG1, IgG2a, IgG2b, IgG2c, and some crossreactivity to rat IgM. The antibody is a F(ab`)2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding.

This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a rat IgG followed by this polyclonal antibody).

Applications Reported: This poly antibody has been reported for use in flow cytometric analysis.

Applications Tested: This poly antibody has been tested by flow cytometric analysis of mouse splenocytes stained with a primary antibody of the rat IgG isotype. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.