Invitrogen™

Dynabeads™ Mouse DC (Dendritic Cell) Enrichment

Use this kit to enrich viable mouse dendritic cells (DC`s) from spleen/lymph node cells by negative isolation. The kit depletes자세히 알아보기

Use this kit to enrich viable mouse dendritic cells (DCs) from spleen/lymph node cells by negative isolation. The kit depletes T cells, mIgM+ B cells, NK cells, erythrocytes and most granulocytes. Highly enriched mouse DCs are bead and antibody-free, and can be used in any functional assay. The cells can be further sorted to obtain high purity of specific DC sub-populations.

Obtain high recovery (80-100%) of viable Lin-CD11c+ DCs of all sup-populations, including DCs positive for CD4, CD8α, B220 and CD19.

• Substantial reduction in sorting time and volume compared to sorting only, thus saving money in addition.
• Pool cells/tissue for easy sample scale -up

Methodology:
An antibody mix towards the non-dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched mouse DC`s can be directly sorted into the sup-population of choice or used in any other application.

Starting samples:
Spleen or lymph node cells.

사양

세포 유형Dendritic Cells

제품 유형Mouse DC Enrichment Kit

분리 기술Negative Isolation

셀 수Processes ∼1 x 109 cells total

출력 생존력>95%

제품라인DYNAL™, Dynabeads™

순도 또는 품질 등급Research Grade

수량5 mL

반응성Mouse

샘플 종류Lymph Node, Spleen

배송 조건Room Temperature

시작 물질 셀 수1 x 107 cells per isolation

타겟 종Mouse

Unit SizeEach