Thermo Fisher Scientific Notch1 Polyclonal Antibody
Rabbit polyclonal antibody targeting human Notch1 (residues 2505–2555). Validated for WB, IHC(P), and IP applications. Supplied as a liquid, unconjugated form at 200 µg/mL. Recommended storage at 4°C and shelf life of 1 year.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:500–1:2,000
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)
- Tested Dilution: 2–10 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 2505 and 2555 of human Notch ortholog 1, translocation-associated |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For Research Use Only. Not for use in diagnostic procedures.
- Not for resale without express authorization.
Target Information
The notch gene belongs to a family of epidermal growth factor (EGF)-like homeotic genes encoding transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3, and Notch4 (Int-3), which are involved in differentiation of the nervous system and other structures.
The EGF-like proteins Delta and Serrate act as ligands for Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) and a transmembrane subunit (Ntm).
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