Thermo Fisher Scientific AHNAK Polyclonal Antibody
AHNAK 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 인간 시료 반응성. 항원 친화 크로마토그래피로 정제된 고품질 항체. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:20–1:50 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human AHNAK. Recombinant protein control fragment (Product #RP-92506). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2637783 |
Product Specific Information
Immunogen sequence:
LGEGHLSVKG SGGEWKGPQV SSALNLDTSK FAGGLHFSGP KVEGGVKGGQ IGLQAPGLSV SGPQGHLESG SGKVTFPKMK IPKFTFSGRE LVGREMGVDV HFPKAEASIQ AGAGDGEWEE SEVKLKKSKI KMPK
Sequence identity:
- Mouse: 87%
- Rat: 84%
Target Information
AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increase in extracellular calcium levels or upon protein kinase C activation. It regulates extracellular calcium influx mediated by L-type Ca²⁺ channels and is involved in signal transduction processes affecting cell differentiation and proliferation.
In response to calcium-dependent intercellular contacts, AHNAK1 forms multimeric complexes in the plasma membrane connected with actin and annexin 2/S100A10 assemblies, contributing to plasma membrane architecture.
In epithelial cells, AHNAK1 is cytoplasmic or membrane-associated, while in non-epithelial cells, it is predominantly nuclear with weak DNA-binding activity and association with the DNA-ligase IV–XRCC4 complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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