Thermo Fisher Scientific PHLDA1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

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View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

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Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PHLDA1. Recombinant protein control fragment (Product #RP-92048).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2645510

Product Specific Information

Immunogen sequence: RMLESSGCKA LKEGVLEKRS DGLLQLWKKK CCILTEEGLL LIPPKQLQHQ QQQQQQQQQQ QQQPGQGPAE PSQPSGPAVA SLEPPVKLKE LHFSNMKTVD CVE

Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 91%.

Target Information

Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an important component of specific effector mechanisms in immunosurveillance against virus-infected or -transformed cells. Two mechanisms appear to account for this activity, one of which is the perforin-based process. Independently, a FAS-based mechanism involves the transducing molecule FAS (APO-1) and its ligand (FAS-L). The human FAS (APO-1) protein is a 48 kDa cell surface glycoprotein that belongs to a family of receptors that includes CD40, nerve growth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, and is expressed at high levels in T cells subsequent to crosslinking of the T cell receptor (TCR). A previously undescribed protein, TDAG51, restores activation- induced apoptosis in cells that have lost the ability to display Fas in response to activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.