Thermo Fisher Scientific NBS1 Monoclonal Antibody (1C3)
NBS1 단백질을 인식하는 Mouse monoclonal antibody로, WB, ICC/IF, IP, ChIP 등 다양한 응용에 사용 가능. Human 및 Mouse 반응성. Protein G 정제, PBS buffer(20% glycerol) 보존. 연구용으로 293T, MCF7, HeLa, Raji 세포에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Immunoprecipitation (IP) | Assay-dependent | - |
| ChIP assay (ChIP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1C3 |
| Immunogen | The complete coding region of the human p95/NBS1 expressed in E. coli. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_560308 |
Product Specific Information
- Recommended positive controls: 293T, MCF7, HeLa, Raji.
Target Information
The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex.
This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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