Thermo Fisher Scientific MRE11 Monoclonal Antibody (12D7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

1:100

-

Functional Assay (Functional)

Assay-dependent

-

in situ PLA (PLA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

12D7

Immunogen

Amino acids 182-582 of Mre11 expressed in E. coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MRE11,uniProtId:P49959-1,ncbiNodeId:9606,antigenRange:182-582,antigenLength:708,antigenImageFileName:MA1-23253_MRE11_P49959-1_House_mouse.svg,antigenImageFileNamePDP:MA1-23253_MRE11_P49959-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.45 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560193

Product Specific Information

Recommended positive controls: HeLa, Jurkat, Raji, NCI-H929, 293T, human Mre11-transfected 293T, PC-12.

Target Information

MRE11 (double-strand break repair protein MRE11) is a component of the MRN complex. This complex plays a central role in double-strand break repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-stranded endonuclease activity and double-strand-specific 3-5 exonuclease activity which are provided by MRE11. RAD50 is required to bind DNA ends and hold them in close proximity. Mutations in the gene could result in ataxia-telangiectasia-like disorder 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.