Thermo Fisher Scientific nNOS Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

1:1500-1:2500

-

Product Specifications

Host/Isotype

Guinea pig / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide from the C-terminal of rat cerebellar NO-synthase, conjugated to BSA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_1077290

Product Specific Information

Reconstitute with 50-100 µL of distilled water, and dilute further in 0.1M PBS with 1% BSA and 0.1% sodium azide.

Target Information

NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.