Thermo Fisher Scientific CD204 Monoclonal Antibody (PSL204), PE-Cyanine7, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.06 µg)/Test

Product Specifications

Additional Formats

• APC
• Request custom conjugation

Product Specific Information

This monoclonal antibody PSL204 reacts with human CD204, also known as Macrophage Scavenger Receptor 1 (MSR1), Scavenger Receptor Type A (SR-A), or SCARA1. It is expressed by multiple populations of macrophages and dendritic cells. CD204 belongs to the class A family of scavenger receptors, a group of five homotrimeric transmembrane proteins with a short cytoplasmic tail and extracellular C-terminus. Other members include MARCO (SCARA2), CSR (SCARA3), SRCL (SCARA4), and SCARA5.

CD204 binds and internalizes oxidized low-density lipoproteins (LDL), influencing macrophage differentiation, cytokine secretion, and atherosclerotic lesion formation. It also binds bacterial components, viral DNA, apoptotic cells, and beta-amyloid fibrils, implicating CD204 in diverse physiological and pathological processes such as host defense, inflammation, bone remodeling, atherosclerosis, sepsis, and Alzheimer’s disease.

In humans, CD204 exists as three splice variants:

• Type I (SR-AI): full-length isoform
• Type II (SR-AII): lacks the C-terminal cysteine-rich domain
• Type III (SR-AIII): shortest form, not expressed on the cell surface

The PSL204 antibody binds only the long (type I, SR-AI) isoform and does not cross-react with mouse or rat CD204.

This antibody has been pre-titrated and tested by flow cytometric analysis of human monocyte-derived macrophages. Use at 5 µL (0.06 µg) per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically (10⁵–10⁸ cells/test).

Light sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD204 gene encodes class A macrophage scavenger receptors (types 1, 2, 3) generated by alternative splicing. These macrophage-specific trimeric membrane glycoproteins are involved in processes such as atherosclerosis, Alzheimer’s disease, and host defense.

• Type 1 and 2 isoforms: Functional receptors mediating endocytosis of modified LDLs
• Type 3 isoform: Trapped in the endoplasmic reticulum, acts as a dominant negative regulator of scavenger receptor activity

Diseases associated with MSR1 include Barrett Esophagus and Prostate Cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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