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It is a selective medium recommended for the simultaneous detection of Escherichia coli and thermotolerant coliforms in water and food samples. The chromogenic mixture contains two chromogenic substrates, which enables the detection of two specific enzymes-galactosidase and ß-glucoronidase. ß-galactosidase produced by coliforms cleaves one chromogen, resulting in the pink colouration of coliform colonies. The enzyme ß-glucuronidase produced by E. coli , cleaves X-glucuronide. Escherichia coli forms dark blue to violet coloured colonies due to cleavage of both the chromogens.
