Carrot Agar
Details
Carrot Agar is recommended for sporangial production and study of mating techniques of phytophthora sp.This medium is widely used in mycological research. Different combinations of carrot and agar are known to support proliferation and sporulation of plant pathogens. It can also be used to enhance sporulation of a variety of fungi such as Alternaria, Cercospora, and Thielaviopsis. This medium has been recommended for studies determining mating type of Phytophthora ramorum isolates by mycelial mixing. Different isolates under study can be subcultured onto this medium until mycelial growth is seen but with no chlamydospores. Such isolates can be grown or subcultured to carry out mating studies at room temperature (20-30°C ) for 3 days as per standard protocol.