Tryptone Lactose Iron Agar
Details
Tryptone Lactose Iron Agar medium is developed for the accurate differentiation and identification of aerobes and anaerobes by means of motility and fermentation reactions and H2S production. It is recommended to study motility of organism and simultaneous sulphite reduction in acidic environment. Due to presence of phenol red in the medium, on fermentation of lactose the medium turns yellow due to production of acid and gas. The ability of an organism to produce H2S is a consistent characteristics and an H2S producer usually blackens the media and produce gas (CO2 + H2) in carbohydrate media, which is visualized as air bubbles in the medium.
