Thermo Fisher Scientific HSP90 Monoclonal Antibody (AC-16), PerCP
HSP90 단백질을 인식하는 AC-16 클론의 PerCP 결합 단일클론 항체. Western blot, IHC, microarray 등 다양한 분석에 사용 가능. 인간, 생쥐 등 여러 종에 반응하며, 단백질 접힘 및 스트레스 반응 연구에 적합. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:2,000 |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Fungi, Human, Insect, Mouse, Plant, Rabbit, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AC-16 |
| Immunogen | Heat shock protein 90 from the water mold Achyla ambisexualis |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931571 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45117 was sufficient for detection of HSP90 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects 90 kDa. This antibody is reactive with both the constitutive and inducible forms of HSP90. It does not bind to the native form and does not recognize HSP90 from E. coli or yeast.
Target Information
HSP90 proteins are highly conserved molecular chaperones involved in signal transduction, protein folding, degradation, and morphologic evolution. They associate with cochaperones to fold newly synthesized proteins or refold denatured proteins after stress.
Major cytosolic forms include HSP90AA1 (inducible) and HSP90AB1 (constitutive), with additional mitochondrial forms (Chen et al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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