Thermo Fisher Scientific ErbB4 (Her4) Monoclonal Antibody (HFR1), Biotin, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Immunoprecipitation (IP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HFR1

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572816

Product Specific Information

Description: The monoclonal antibody HFR1 recognizes human and mouse epidermal growth factor receptor 4 (ErbB4 or HER4). ErbB4 belongs to the family of receptor tyrosine kinases (RTK) of which HER1 (EGRF), HER2 (ErbB2), and HER3 (ErbB3) are members. RTKs function to affect a variety of cell processes including proliferation, differentiation, migration, survival and apoptosis and are often disregulated in cancer. The ErbB4 protein contains extracellular ligand binding domains, a transmembrane region, and a cytoplasmic tail containing the tyrosine kinase domain and the binding site for the HFR1 antibody. Upon ligand binding, ErbB4 homo- or hetero-dimerizes with other family members, becomes phosphorylated, and can be cleaved allowing translocation to the nucleus. The HRF1 antibody recognizes membrane-bound, cytoplasmic and nuclear ErbB4. Cytosolic ErbB4 expression was found to correlate with a positive prognosis in a subset of breast cancer patients, while nuclear ErbB4 expression was inversely correlated with tumor grade and aggressiveness of breast tumors.

Applications Reported: This HFR1 antibody has been reported for use in immunoprecipitation, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This HFR1 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. ErbB 4 is activated by neuregulins and NTAK (neural and thymus derived activator for ErbB kinases). ErbB 4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Like other protein kinases, ErbB 4 mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3. ErbB 4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.