Thermo Fisher Scientific IL-8 (CXCL8) Polyclonal Antibody, Biotin, PeproTech
Human IL-8(CXCL8)에 특이적인 Biotin 결합 Rabbit Polyclonal 항체로, Western blot 및 ELISA에 최적화됨. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 감도를 제공. 연구용으로 염증 반응 및 세포 신호 연구에 활용 가능.
- 카탈로그번호
- 500-P28BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-8 (CXCL8) Polyclonal Antibody, Biotin, PeproTech
Thermo Fisher Scientific IL-8 (CXCL8) Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | — |
| ELISA | 0.25–1.0 µg/mL | 12 publications |
| In vitro Assay (IV) | — | 1 publication |
| Miscellaneous PubMed | — | 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-8 (CXCL8) (72 a.a.) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930255 |
Product Specific Information
AA Sequence of Recombinant Protein:
SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-8 (CXCL8). Anti-Human IL-8 (CXCL8)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human IL-8 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-8 (500-P28) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human IL-8.
Western Blot:
To detect Human IL-8 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
When used with compatible secondary reagents, the detection limit for Recombinant Human IL-8 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Packaging:
500-P28BT-1MG will be provided as 2 × 500 µg.
Target Information
Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor belonging to the CXC chemokine subfamily. It binds to cell surface receptors IL-8RA and IL-8RB and functions as a chemoattractant and angiogenic factor.
IL-8 expression and secretion are induced by inflammatory stimuli in many cell types, including macrophages and endothelial cells. In endothelial cells, IL-8 resides in Weibel-Palade bodies.
IL-8 signals through CXCR1 and CXCR2 receptors and was previously known as NAP-1, GCP-1, MONAP, and protein 3-10C.
IL-8 and ten other CXC chemokines form a gene cluster on chromosome 4q.
Studies suggest IL-8 plays a role in tumor angiogenesis and viral bronchiolitis pathogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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