Thermo Fisher Scientific Methylglyoxal Monoclonal Antibody (9E7), APC
Methylglyoxal 변형 단백질을 특이적으로 인식하는 마우스 단클론 항체. APC 형광 표지로 Flow Cytometry 및 Immunofluorescence에 적합. Western blot, IHC 등 다양한 응용 가능. 보존제 무첨가, 단백질 G 정제, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25–1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9E7 |
| Immunogen | Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | APC (Allophycocyanin) |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932263 |
Available Formats
- Unconjugated (MA5-27567)
- FITC (MA5-45810)
- PE (MA5-45812)
- PerCP (MA5-45811)
- Request custom conjugation
Product Specific Information
A 1:1,000 dilution of MA5-45809 was sufficient for detection of Methylglyoxal in 0.5 µg of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal.
Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
Target Information
Methylglyoxal (also called pyruvaldehyde or 2-oxopropanal) is an organic compound with the formula CH₃C(O)CHO. Gaseous methylglyoxal has two carbonyl groups (an aldehyde and a ketone), but in the presence of water, it exists as hydrates and oligomers. It is a reduced derivative of pyruvic acid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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