Thermo Fisher Scientific CD91 Monoclonal Antibody (A2MR-a2), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.05 µg)/test

Product Specifications

Additional Formats

• eFluor 660
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 725
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation

Product Specific Information

The A2MR-a2 monoclonal antibody reacts with human CD91 (also known as LRP-1 or alpha-2 macroglobulin receptor).
CD91 is a 600 kDa type I transmembrane protein composed of alpha and beta chains. The alpha chain binds diverse ligands, while the beta chain contains tyrosine phosphorylation motifs that interact with adaptor and signaling proteins.
Ligand binding mediates endocytosis and lysosomal trafficking. CD91 is expressed by monocytic cells, dendritic cells, hepatocytes, fibroblasts, adipocytes, neurons, and syncytiotrophoblasts. It is involved in antigen cross-presentation via MHC class I and in signaling cascades related to cell migration.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on normal human peripheral blood cells at 5 µL (0.05 µg)/test.
A test is defined as the amount of antibody staining a sample in 100 µL volume (10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures for optimized fluorescence and minimal spillover.

Excitation: 639 nm
Emission: 710 nm
Laser: 633–640 nm (Red)

Target Information

The encoded CD91 protein functions as an endocytic receptor involved in intracellular signaling, lipid homeostasis, and apoptotic cell clearance.
It is essential for A2M-mediated clearance of amyloid precursor protein and beta-amyloid, the main component of Alzheimer’s plaques.
Expression decreases with age and is reduced in Alzheimer’s brain tissue compared to controls.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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