Thermo Fisher Scientific H4K20me1 Monoclonal Antibody (5E10-D8), ChIP-Verified
H4K20me1 단일메틸화 히스톤 H4에 특이적인 Thermo Fisher Scientific의 ChIP-검증 모노클로날 항체입니다. Western blot, ChIP, IHC, ICC/IF 등 다양한 응용에 사용 가능하며, 단일메틸화 상태만 인식하고 다른 메틸화 상태와 교차반응하지 않습니다. Human 반응성, Mouse IgG1 형식입니다.
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Thermo Fisher Scientific H4K20me1 Monoclonal Antibody (5E10-D8), ChIP-Verified
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | Assay-dependent | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| ELISA | Assay-dependent | - |
| ChIP assay (ChIP) | 1–5 µg | View 1 publication |
| Dot blot (DB) | Assay-dependent | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5E10-D8 |
| Immunogen | Synthetic peptide within Human Histone H4 (mono methyl K20) (Sequence: 15-AKRHR(Kme)VLRDN-25 (branched)) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2866501 |
Product Specific Information
This antibody is specific for K20 mono-methylated histone H4 and does not cross-react with histone 3 or tri-, di-, or unmethylated histone H4.
Predicted to react with Xenopus laevis, Arabidopsis thaliana, Drosophila melanogaster, and most mammals based on sequence homology.
- Predicted molecular weight: 11 kDa
- Observed band (WB): 13 kDa
- Blocking peptide: mono-methyl K20 Histone H4 peptide
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Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fiber in eukaryotes.
Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and functions in chromatin compaction.
This gene is intronless and encodes a member of the histone H4 family, located in the large histone gene cluster on chromosome 6.
Transcripts lack polyA tails but contain a palindromic termination element.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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