Thermo Fisher Scientific CD14 Monoclonal Antibody (MEM-18), Biotin
CD14 단백질을 인식하는 Biotin 결합 마우스 단클론 항체로, 인간 및 비인간 영장류 시료 분석에 적합합니다. Western blot, Flow cytometry, ELISA, Immunoprecipitation 등 다양한 응용에 사용 가능합니다. 1 mg/mL 농도의 액상 형태로 제공되며, 4°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Flow Cytometry (Flow) | 2–4 µg/mL |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MEM-18 |
| Immunogen | Human urinary proteins from the urine of a patient suffering from proteinuria |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1071265 |
Additional Formats
Product Specific Information
This antibody reacts with CD14, a 53–55 kDa GPI-linked extracellular membrane glycoprotein expressed on monocytes, macrophages, and weakly on granulocytes; also expressed by most tissue macrophages. In humans, the epitope recognized by MEM-18 is located between amino acids 57–64.
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. It functions as a multifunctional lipopolysaccharide receptor and exists in both membrane-bound and soluble forms. CD14 binds lipopolysaccharides in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), contributing to immune defense mechanisms. It also binds apoptotic cells and influences allergic, inflammatory, and infectious processes. Alternative splicing produces multiple transcript variants encoding the same isoform. CD14 dysfunction is associated with conditions such as Mycobacterium chelonae infection and croup.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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