
Thermo Fisher Scientific Cytokeratin 19 Monoclonal Antibody (A53-B/A2), Biotin
Cytokeratin 19 단백질을 인식하는 Biotin 결합 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. 인간 시료에 반응하며, rod domain을 인식합니다. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | 1 publication |
| Flow Cytometry (Flow) | 1–5 µg/mL | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A53-B/A2 |
| Immunogen | Human mammary adenocarcinoma cell line MCF-7 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1075173 |
Additional Formats
Product Specific Information
This antibody recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes and is often co-expressed with cytokeratin 7.
Target Information
Cytokeratin 19 belongs to a subfamily of intermediate filament proteins characterized by biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40–68 kDa and isoelectric point from 4.9–7.8.
Cytokeratin subtype expression patterns are used to distinguish different epithelial malignancies. Cytokeratin antibodies aid in tumor differential diagnosis using immunohistochemistry, cytopathology, and flow cytometry.
Notably, cytokeratin 19 is not expressed in hepatocytes, making it useful for identifying liver metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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