Thermo Fisher Scientific c-Myc Chimeric Recombinant Mouse Monoclonal Antibody (9E10)

Thermo Fisher Scientific c-Myc Chimeric Recombinant Mouse Monoclonal Antibody (9E10)

Applications

• Western Blot (WB): Assay-dependent
• Immunohistochemistry (Paraffin) (IHC (P)): Assay-dependent
• Immunohistochemistry (Frozen) (IHC (F)): Assay-dependent
• Immunocytochemistry (ICC/IF): Assay-dependent
• Immunoprecipitation (IP): Assay-dependent

Product Specifications

Product Specific Information

Epitope sequence: EQKLISEEDL, located in aa 410–419 of human c-myc protein.

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays a crucial role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.

Phosphorylation of c-Myc at Thr58/Ser62 by enzymes such as glycogen synthase kinase 3, cyclin-dependent kinase, ERK2, and JNK is associated with cell proliferation and cycle regulation. Studies indicate c-Myc is essential for vasculogenesis and angiogenesis during tumor development.

The c-myc oncogene (p62 c-myc) regulates normal cellular proliferation and differentiation. Deregulated expression can induce apoptosis in multiple cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tags fused to amino or carboxy termini of target proteins.

c-Myc-, N-Myc-, and L-Myc-encoded proteins are involved in cell proliferation and neoplastic diseases. Amplification of the c-Myc gene is found in human tumors such as lung, breast, and colon carcinomas, while N-Myc amplification is common in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to immunoglobulin loci on chromosomes 14, 2, or 22 is characteristic of Burkitt’s lymphoma and other B-cell lymphoproliferative conditions.

Aberrant expression of the c-myc gene occurs in tumors of diverse origins including colorectal, gastric, hepatic, mammary, ovarian, pulmonary, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.