Thermo Fisher Scientific c-Myc Chimeric Recombinant Human Monoclonal Antibody (9E10)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Epitope sequence: EQKLISEEDL, located in aa 410–419 of human c-Myc protein.

Target Information

The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is frequently activated in various tumor cells and plays a key role in cellular proliferation, differentiation, apoptosis, and cell cycle progression.

Phosphorylation of c-Myc at Thr58/Ser62 by kinases such as glycogen synthase kinase 3, cyclin-dependent kinase, ERK2, and JNK has been linked to cell proliferation and regulation. c-Myc is essential for vasculogenesis and angiogenesis during tumor development.

The c-myc oncogene (p62 c-myc) regulates normal cell proliferation and differentiation. Deregulated expression can induce apoptosis in multiple cell types. Antibodies against c-Myc epitopes recognize overexpressed proteins containing Myc epitope tags fused to target proteins.

Amplification of the c-Myc gene has been observed in human tumors, including lung, breast, and colon carcinomas, while N-Myc amplification is associated with neuroblastomas. Translocation of the c-Myc locus on chromosome 8 to immunoglobulin loci on chromosomes 14, 2, or 22 is characteristic of Burkitt’s lymphoma and other B-cell proliferative disorders. Aberrant c-Myc expression is found in diverse tumors such as colorectal, gastric, hepatic, mammary, ovarian, and hematopoietic neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.