Thermo Fisher Scientific CD235a (Glycophorin A) Chimeric Recombinant Rabbit Monoclonal Antibody (M2A1)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Inhibition Assays (IA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

M2A1

Immunogen

M2A1 was prepared by immunzing BALB/c mice with human red blood cells of the M phenotype, and was screened by testing for anti-M activity. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD235a (Glycophorin A),uniProtId:P02724-1,ncbiNodeId:9606,antigenRange:1-150,antigenLength:150,antigenImageFileName:MA5-48073_CD235a_Glycophorin_A_P02724-1_Rabbit.svg,antigenImageFileNamePDP:MA5-48073_CD235a_Glycophorin_A_P02724-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074516

Product Specific Information

Specificity: M2A1 binds specifically to the M antigen on human erythrocytes, and shows no reactivity to N antigen. The epitope for this antibody includes the amino-terminal serine residue and sialic acid residues of glycophorin A. The reactivity of M2A1 for M antigen is pH- and salt-dependent (optimum ~ pH8-9). Blood group antigen M is located on the major sialoglycoprotein (glycophorin A) of red blood cells.

Target Information

CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.