Thermo Fisher Scientific NSE Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Suggested positive control: antigen standard for ENO2 (transient overexpression lysate), rat spinal cord or peripheral nerve homogenate, and HEK 293 lysate.

Target Information

Neuron specific enolase (NSE, also known as ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis. NSE is highly stable in biological fluids and diffuses into extracellular media and cerebrospinal fluid (CSF) upon neuronal injury.

NSE is one of three mammalian enolases (α, β, γ subunits). The α-subunit is expressed in most tissues, β-subunit in muscle, and γ-subunit primarily in neurons and neuroendocrine cells. Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms.

Antibodies to NSE are useful for identifying neuronal cell bodies, developing neuronal lineages, and neuroendocrine cells. Release of NSE into CSF and blood serves as a biomarker for neuronal injury. Clinically, NSE is used as a sensitive marker in neurological disorders such as stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Elevated NSE levels are also found in plasma and certain neoplasias such as pediatric neuroblastoma and small cell lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.