Thermo Fisher Scientific Phospho-Dynamin 1 (Ser774) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:1,000

-

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 of rat DNM1 conjugated to KLH if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Dynamin 1,uniProtId:P21575-1,ncbiNodeId:10114,antigenRange:774,antigenLength:864,antigenImageFileName:PA1-4620_Dynamin_1_P21575-1_Sheep.svg,antigenImageFileNamePDP:PA1-4620_Dynamin_1_P21575-1_Sheep_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560610

Product Specific Information

This antibody is predicted to react with bovine, canine, chicken, human, mouse and non-human primate based on 100% sequence homology. It does not cross-react with other purified substrates of cdk5 (amphiphysin and synapsin).

This antibody is specific for the ~95 kDa Dynamin protein phosphorylated at Ser774in Western blots of rat brain lysates. This antibody labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Store at -20ºC short term, 80ºC long term.

Target Information

Dynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin`s involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.