Thermo Fisher Scientific Ryanodine Receptor Monoclonal Antibody (C3-33)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 106 publications 106 publications

Immunohistochemistry (IHC)

-

View 30 publications 30 publications

Immunohistochemistry (Paraffin) (IHC (P))

1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:50

View 41 publications 41 publications

Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

View 7 publications 7 publications

ChIP assay (ChIP)

-

View 1 publication 1 publication

in situ PLA (PLA)

-

View 1 publication 1 publication

Dot blot (DB)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Amphibian, Dog, Chicken, Fish, Guinea pig, Human, Mouse, Rabbit, Rat

Published species

Dog, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

C3-33

Immunogen

Canine cardiac ryanodine receptor (RyR2)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2183054

Product Specific Information

MA3-916 detects ryanodine receptor 2 (RyR2) and shows cross-reactivity with RyR1 in amphibian, canine, chicken, fish, guinea pig and rat tissues.

MA3-916 has been successfully used in Western blot, immunohistochemical, immunofluorescence, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 565 kDa protein representing the ryanodine receptor. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor. Immunohistochemical staining of RyR in rat cardiac tissue with MA3-916 results in intense staining of the myofiber, which is consistent with sarcoplasmic reticulum localization.

Target Information

The ryanodine receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the foot region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.