Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), Biotin, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The 390 monoclonal antibody reacts with mouse CD31 (platelet-endothelial cell adhesion molecule-1, PECAM-1, gpIIa). This 130–140 kDa surface protein is expressed by endothelial cells and at low levels on all leukocytes and platelets. CD31 participates in adhesion, cell-cell and cell-matrix interactions, and signal transduction.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse thymocytes and splenocytes, ≤0.25 µg/test in 100 µL volume. Optimal antibody titration is recommended.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling through ITIM motifs that recruit protein-tyrosine phosphatases upon phosphorylation. It is expressed throughout the vascular system, mainly at intercellular junctions. The N-terminal Ig-like domain mediates homophilic binding, critical for cell-cell interaction.

CD31 functions in:

• Integrin-mediated adhesion
• Transendothelial migration
• Angiogenesis
• Apoptosis regulation
• Negative regulation of immunoreceptor signaling
• Autoimmunity and macrophage phagocytosis
• IgE-mediated anaphylaxis and thrombosis

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.