Thermo Fisher Scientific CD40 Monoclonal Antibody (1C10), Biotin, eBioscience
마우스 CD40 단백질을 인식하는 Biotin 결합 단클론 항체로, Flow Cytometry 및 ELISA에 적합합니다. B 세포 발달 및 면역 조절 연구에 활용되며, 1C10 클론은 in vitro/in vivo에서 작용 활성이 보고되었습니다. 4°C 암소 보관, 동결 금지.
- 카탈로그번호
- 13-0401-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD40 Monoclonal Antibody (1C10), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 1 µg/test | View 33 publications |
| ELISA | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1C10 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466431 |
Additional Formats
- Unconjugated
- APC
- Brilliant UV 661
- Brilliant Violet 605
- Functional Grade
- PE
- PE-Cyanine5
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Request custom conjugation
Product Specific Information
The 1C10 monoclonal antibody reacts with mouse CD40, a 45–50 kDa type I transmembrane glycoprotein belonging to the TNFR family. CD40 is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. It regulates B cell development and maturation, induces Ig isotype switching, and promotes proliferation while preventing apoptosis in combination with IL-4. Interaction with CD154 (gp39) on T cells is critical for T-B cell crosstalk and immune regulation.
The 1C10 clone has been reported to exhibit agonistic activity both in vitro and in vivo.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤1 µg/test (100 µL final volume). Optimal titration recommended.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD40 is a single-chain glycoprotein and a member of the TNF/NGF receptor superfamily. It is expressed on all B cells except plasma cells and also on epithelial cells, carcinomas, and lymphoid dendritic cells. CD40 mediates immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
The transcription factor AKNA regulates CD40 expression, while TNFR2 acts as an adaptor protein in CD40 signaling. CD40-CD154 interaction is implicated in amyloid-beta-induced microglial activation, an early event in Alzheimer’s disease. Mutations in CD40 are associated with Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.