Thermo Fisher Scientific alpha Internexin Monoclonal Antibody (2E3)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

1-5 µg/mL

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rabbit, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2E3

Immunogen

Full-length recombinant rat alpha-internexin protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Internexin,uniProtId:P23565-1,ncbiNodeId:10114,antigenRange:1-505,antigenLength:505,antigenImageFileName:32-3600_alpha_Internexin_P23565-1_House_mouse.svg,antigenImageFileNamePDP:32-3600_alpha_Internexin_P23565-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533075

Product Specific Information

This antibody is purified from mouse ascites fluid and is specific for the 66 kDa alpha-internexin protein. 2E3 antibody has stained human neuron and nerve fiber in human cerebral cortex. It has also stained glioma, ganglioneuroma, pheochromocytoma, and paraganglioma in human tissue. It is recommended that FFPE tissues are pretreated with HIER using citrate buffer, pH 6.

Target Information

alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.