Thermo Fisher Scientific beta Tubulin Monoclonal Antibody (2 28 33)
다양한 종에서 반응하는 베타 튜불린 단클론 항체로, Western blot 및 면역형광 등 다양한 응용에 적합. 비결합형 포맷으로 제공되며, 0.5 mg/mL 농도의 액상 형태. 단백질 A로 정제되어 높은 특이성과 재현성 제공. 연구용 전용 제품.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | View 74 publications |
| Immunohistochemistry (IHC) | – | View 4 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 1 publication |
| Immunohistochemistry - Free Floating (IHC (Free)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1–3 µg/mL | View 14 publications |
| Flow Cytometry (Flow) | – | View 1 publication |
| Immunoprecipitation (IP) | – | View 2 publications |
| Miscellaneous PubMed (Misc) | – | View 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, C. elegans, Human, Mouse, Non-human primate, Rat |
| Published Species | C. elegans, Dog, Human, Mouse, Non-human primate, Pig, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2 28 33 |
| Immunogen | Beta-tubulin from sea urchin (S. purpuratus) sperm |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533072 |
Available Conjugated Formats
Product Specific Information
This antibody reacts with the ~50 kDa beta-tubulin and binds to two major and one minor beta-tubulin isotypes. Reactivity has been confirmed with mouse NIH3T3 fibroblast cells, rat brain, and mouse testis.
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene cause congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin is also detected in Sertoli cells and transiently in non-neuronal embryonic tissues. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, which inhibits its incorporation into microtubules. Microtubules, composed of alpha and beta tubulin dimers, play key roles in mitosis, intracellular transport, and cytoskeletal structure. Beta-tubulin is ubiquitously expressed and commonly used as a loading control in protein detection assays such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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