
Thermo Fisher Scientific Rat IgG2a kappa Isotype Control (eBR2a), eFluor 506, eBioscience
Flow cytometry용 Rat IgG2a kappa 동종 대조 항체로, eFluor 506 형광체 결합. 405 nm 레이저로 여기되어 506 nm에서 방출. 실험 항체와 동일 농도로 사용 권장. 0.2 mg/mL 농도, 4°C 암소 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBR2a |
| Conjugate | eFluor™ 506 |
| Excitation/Emission Max | 419 / 508 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637338 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 421–786
- eFluor 450, 570, 615, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Functional Grade
- Pacific Orange
- Custom conjugation available upon request
Product Specific Information
The monoclonal rat IgG2a is useful as an isotype control immunoglobulin.
This eBR2a antibody has been reported for use in flow cytometric analysis.
It has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes.
Use the isotype control at the same concentration as the experimental antibody.
eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm.
Recommended filter: 510/20 band pass filter or equivalent.
Ensure that the instrument can detect this fluorochrome.
- Excitation: 405 nm
- Emission: 506 nm
- Laser: Violet Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
Rat IgG2a is an immunoglobulin subclass commonly used in research and diagnostics.
It is produced by the rat immune system, capable of activating complement and mediating ADCC.
Molecular weight: approximately 160 kDa (two heavy chains and two light chains).
Used as a control or reference antibody in rat-based experiments, flow cytometry, and immunohistochemistry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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