Thermo Fisher Scientific TCF4/TCF12 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to amino acids 580-630 of human TCF-4 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TCF4/TCF12,uniProtId:P15884-1,ncbiNodeId:9606,antigenRange:580-630,antigenLength:667,antigenImageFileName:PA5-36151_TCF4TCF12_P15884-1_Rabbit.svg,antigenImageFileNamePDP:PA5-36151_TCF4TCF12_P15884-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2553395

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 60 and 72 kDa.

Purity is >95% by SDS-PAGE.

Target Information

T cell factors (TCFs) comprise a family of DNA-binding transcriptional activators that are essential for lymphoid cell development. These transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group(HMG) 1 box, which mediates DNA binding. TCF-4 mainly localizes in the cytoplasm and is transported into the nucleus directly bound to beta-catenin in a cooperative manner. This TCF-4/ beta-catenin complex induces expression of Wnt target genes, including multiple cancer-associated genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.