Thermo Fisher Scientific MYLK Polyclonal Antibody
MYLK 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 IHC, ICC/IF에 사용 가능. Human과 Mouse에 반응하며, KLH 결합 합성 펩타이드로 면역생성. 연구용으로 세포 신호 및 근육 수축 관련 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 923–953 amino acids from the N-terminal region of human MLCK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Ammonium sulfate precipitation, Size-exclusion, Dialysis |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2298066 |
Product Specific Information
This antibody is predicted to react with rabbit based on sequence homology.
Target Information
MYLK (MLCK) is a serine/threonine kinase that phosphorylates myosin regulatory light chains to assist in contractile activity (myosin-actin interaction).
MLCK mediates smooth muscle contraction via phosphorylation of a specific serine residue in the N-terminus of myosin light chains (MLC), facilitating myosin interaction with actin filaments.
It plays a key role in vascular permeability, tissue edema formation, and in the nervous system contributes to astrocytic process growth and neurotransmitter release.
MLCK also participates in fibroblast apoptosis signaling.
Smooth muscle and non-muscle isozymes are expressed broadly across adult and fetal tissues.
Non-muscle isoform 2 is the dominant splice variant, and the Telokin isoform binds calmodulin.
MLCK is likely down-regulated by phosphorylation and contains one fibronectin type III domain and nine immunoglobulin-like C2-type domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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