Thermo Fisher Scientific ARAF Monoclonal Antibody (3E2)
ARAF 단백질을 인식하는 Mouse monoclonal antibody (Clone 3E2). Western blot, ELISA, in situ PLA에 사용 가능. Human 시료 반응성. Affinity chromatography로 정제된 액상 항체로, PBS(pH 7.4)에 보존제 없이 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| ELISA | 0.1 ng/mL |
| in situ PLA (PLA) | 1:1200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3E2 |
| Immunogen | ARAF (NP_001645, 151 a.a. approximately 250 a.a) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein:
RQQFYHSVQD LSGGSRQHEA PSNRPLNELL TPQGPSPRTQ HCDPEHFPFP APANAPLQRI RSTSTPNVHM VSTTAPMDSN LIQLTGQSFS TDAAGSRGGS
Target Information
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity and is broadly expressed in nearly all cell lines tested. It is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. Unregulated kinase activity of v-Raf is associated with transformation and mitogenesis, while Raf-1 activity is normally suppressed by its regulatory N-terminal domain.
A-Raf, a second member of the Raf gene family, shares substantial homology with Raf-1 within the kinase domain but less elsewhere. A-Raf expression is highest in urogenital tissues and kidney, and lowest in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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