Thermo Fisher Scientific Tau Monoclonal Antibody (HT7), Biotin

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 32 publications 32 publications

Immunohistochemistry (IHC)

Assay-dependent

View 23 publications 23 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 5 publications 5 publications

ELISA (ELISA)

Assay-dependent

View 21 publications 21 publications

Immunoprecipitation (IP)

-

View 4 publications 4 publications

Luminex (LUM)

-

View 1 publication 1 publication

Inhibition Assays (IA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Non-human primate

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

HT7

Immunogen

Purified human Tau, epitope human Tau between residue 159 and 163 (numbering according to human Tau40), corresponding to the amino acid sequence PPGQK. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Tau,uniProtId:P10636-1,ncbiNodeId:9606,antigenRange:159-163,antigenLength:758,antigenImageFileName:MN1000B_Tau_P10636-1_House_mouse.svg,antigenImageFileNamePDP:MN1000B_Tau_P10636-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Request custom conjugation

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_223453

Product Specific Information

MN1000B targets Tau in ELISA, WB and IHC applications and shows reactivity with Bovine, and Human samples.

The MN1000B immunogen is purified human Tau, epitope human Tau between residue 159 and 163 (numbering according to human Tau40), corresponding to the amino acid sequence PPGQK.

MN1000B detects Tau which has a predicted molecular weight of approximately 79 kDa.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimers diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Picks disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.