Thermo Fisher Scientific beta Actin Recombinant Mouse Monoclonal Antibody (A2-F6-R)
Recombinant mouse monoclonal antibody against beta actin for use in Western blot. Recognizes human, mouse, and rat beta-actin. High specificity and sensitivity with dilution range 1:5,000–1:80,000. Suitable as loading control for protein assays.
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Applications
- Western Blot (WB)
Tested Dilution: 1:5,000–1:80,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | A2-F6-R |
| Immunogen | Synthetic peptide within N-terminal human Beta-actin (aa 1–50/375) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Wet ice |
| RRID | AB_3094326 |
Product Specific Information
- Positive control: HepG2, NIH/3T3, PC-12, MCF-7, THP-1, PMVEC, RH-35, RAW264.7, B16F1, MEF, HeLa, F9, Mouse lung, L6 cell lysates
- Predicted band size: 42 kDa
- Subcellular location: Cytoplasm
Target Information
Beta actin is one of six actin isoforms identified in humans. It is a non-muscle cytoskeletal protein expressed in all cell types and plays a key role in cell motility, structure, and integrity. Beta and gamma actin coexist as cytoskeletal components in most cell types. Polymerization of globular actin (G-actin) forms filamentous actin (F-actin) with a two-stranded helical structure. Due to its ubiquitous expression, beta actin is commonly used as a loading control in protein detection assays such as Western blotting. Antibodies against beta actin are valuable tools for studying intracellular distribution and cytoskeletal dynamics.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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