Thermo Fisher Scientific Caspase 1 Polyclonal Antibody
Human Caspase 1 단백질을 인식하는 Rabbit Polyclonal Antibody로, IHC(P)에서 1:50 희석 비율로 사용 가능. 다양한 종에 반응 예측됨. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반 저장용액 포함. 연구용으로만 사용.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1:50
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from internal region of human caspase 1 protein (aa 200–404) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2290752 |
Product Specific Information
This antibody is predicted to react with bovine, canine, mouse, porcine, and rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is placenta tissue.
Target Information
Caspases are a family of cysteine proteases divided into apoptotic and inflammatory subfamilies.
Inflammatory caspases (such as caspase-1, -4, -5, and -12) are associated with immune responses to microbial pathogens and can activate proinflammatory cytokines such as IL-1β and IL-18.
Caspase-1 was initially identified as an IL-1β-converting enzyme and later recognized as a mammalian homolog of the C. elegans cell death gene ced-3, whose overexpression induces apoptosis in fibroblasts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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